Biologist

Biologist

Duration: 6 month contract

Location: Onsite in Boston, MA

Pay Range: $75.00 - $88.00

Hours: 8:30am – 5:00pm Monday to Friday

Job Requirements

Responsibilities:

Conduct experiments to identify and validate drug targets. Support assay design, optimization, validation, and data analyses. Design and execute molecular ,cell biology and biochemical experiments. Troubleshoot and analyze complex data in support of drug discovery projects. Presentation of results and conclusions both orally and written.

We are seeking a skilled, motivated, and versatile individual to join a highly collaborative, successful drug discovery and development team within the CMR group in Boston. The ideal candidate should possess a solid background in cell culture and cell-based functional assays, playing an essential role in advancing our discovery initiatives and pipeline projects centered on anti-obesity therapeutics. In this role, the candidate will support assay development and perform established assays to validate new targets and characterize the in vitro functions of novel therapeutics. Furthermore, the candidate will handle setting up essential laboratory capabilities and work with other scientists both within and across departments to meet project and departmental objectives.

Key position requirements/responsibilities include:

Technical/scientific skills. Expertise in the following is a must-have:

Aseptic cell culture techniques with mammalian cells.

Transient transfections and generation of stable cell lines.

Development of cell-based functional assays, biochemical and enzymatic assays and screening of novel therapeutics in 96-/384-well plate formats at a single concentration or in a concentration/response manner.

Knowledge of basic pharmacology (e.g. concentration/response curves, determination of binding affinity, potency, Hill’s slope and in vitro efficacy). Mechanism of action studies of drugs.

Experience with colorimetric, luminescence and fluorescence-based readouts, including HTRF. Proficient in operating plate readers, liquid handling robots and assay automation.

Design, develop, and optimize in vitro enzymatic assays e.g., ADP-Glo, Kinase-Glo assays

Gene expression studies by qPCR, western blot and FACS techniques.

Soft skills:

Proficient oral and written communication skills and ability to work both independently and in teams.

Requirement for accurate record keeping and independent data analysis. Will perform work in full compliance with Lilly Research Policies and complete all required training activities in the timeframe specified.

Attention to detail and ability to prioritize work on multiple projects, timelines and company goals.

Eager to grasp fundamental principles, swiftly acquire new abilities, and adjust to novel challenges.

Minimum qualifications:

B.S. and/or M.S. in biology, pharmacology, biochemistry or related field.

8-10 years of laboratory experience.

Additional Skills/Preferences

Experience/expertise with the following is a plus:

Native cell lines and primary cell cultures, iPSCs,.

Industry experience.

Exposure to multiple therapeutic target classes (e.g. membrane receptors, including G-Protein Coupled Receptors or GPCRs, enzymes, transporters, etc.) and multiple modalities (small molecules, peptides, antibodies, siRNAs, etc.).

Experience with ELISA assays.

Working knowledge or background in cardiometabolic diseases.

Benefits (employee contribution):

• Health insurance
• Health savings account
• Dental insurance
• Vision insurance
• Flexible spending accounts
• Life insurance
• Retirement plan

All qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. Rate of pay within the stated range will depend on the qualification of the applicant.