Junior Specialist- Delling Lab

The Department of Physiology is now recruiting a Junior Specialist. The area of specialization/responsibilities include:

• Cell culture techniques: working under sterile conditions, cell transfection, viral infection, cell freezing and thawing, optimization of cell culture conditions, live imaging.
• Animal care: mouse husbandry, genotyping of transgenic lines.
• Prepare and track purchasing and payment authorizations.
• Molecular biology techniques: DNA extraction from animal tissue, Genotyping, design of PCR primers, Western blotting, RNA isolation from animal tissue, restriction enzyme digestion and mapping, agarose gel electrophoresis, isolation of DNA from agarose gels, ligation of DNA, transformation of DNA, mini- and maxi-preparation of DNA, site-directed DNA mutagenesis
• General laboratory skills: making solutions, calculating molarity, percent solutions (v/v, w/v), correct use of pipettes, pipettemen, and other liquid transfer devices.

Required Qualifications:

• BA/BS Degree. Degree must be in Biological Sciences with emphasis on cellular and/or molecular biology
• Basic laboratory skills: Molarity calculations, accurate solution formulation, pH determination, maintenance of accurate notebooks, ability to understand and follow basic laboratory protocols with direct supervision, ability to perform complex tasks without committing errors.
• Molecular biology: Laboratory experience including experience with each of the following: PCR, DNA isolation and manipulation, PCR genotyping interpretation, protein gel electrophoresis
• Candidates must meet the required qualifications at the time of appointment. Candidate's CV must state basic qualifications (or if pending) upon submission.

Please apply online at https://aprecruit.ucsf.edu/JPF04610.

See Table 24B for the salary range for this position. A reasonable estimate for this position is $49,000-$52,200.

As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.

UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.