The Tsibris Lab is looking for a highly motivated person to join us in studies of HIV latency and the development of novel approaches to cure HIV. The anticipated earliest start date for this position is April - June 2024. Projects include studies of virus reservoir dynamics and novel strategies to eradicate latent HIV. Virus work may involve HIV-1, HIV-2, and/or HTLV-1. The ideal candidate will be motivated to study the biology of the immune system and retroviral pathogens. Approaches that we use to address these issues include molecular biology, virology, cell and tissue culture, drug susceptibility assays, flow cytometry, deep sequencing, and integrated single-cell analyses. The applicant will work under the Principal Investigator's general supervision, perform duties per established hospital policies and procedures, and conduct various experiments with established methodologies. A 2-year commitment is preferred.

All skills will be taught, but some prior experience with laboratory techniques, such as molecular biology, cell biology, and sterile technique, is essential.

This position is at 65 Landsdowne Street, Cambridge, MA.

QUALIFICATIONS:

* BS OR BA

* New graduate with relevant course or project work

PRINCIPAL DUTIES AND RESPONSIBILITIES

1. Under general supervision, performs a variety of routine tests following established methodologies. Routine tests may include virus RNA extraction, PCR, DNA sequencing, real-time PCR, plasma and PBMC isolation from whole blood, PBMC subpopulation isolation, and drug susceptibility assays with HIV-1 and HIV-2.

2. Prepares and maintains cell cultures and virus stocks. This work will include quantitative virus outgrowth assays and some work with HIV-infected human tissues.

3. Records data, summarizes, and analyzes results using basic statistics.

4. Prepares laboratory reagents, chemicals, instruments, and equipment.

5. Maintains supplies inventory in accordance with departmental procedures.

6. Attends weekly lab meetings and discusses the progress of work regularly with a supervisor.

7. All other duties as assigned.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:

1. Interested in a career in biomedical research

2. Strong organizational skills, including the ability to handle a variety of tasks simultaneously

3. Sound degree of computer literacy

4. Ability to record and analyze data using basic statistics

5. Ability to perform a variety of routine tests and prioritize work.

WORKING CONDITIONS:

Works in BL2 laboratory. Interacts collegially with other lab members under the supervision of the Principal Investigator and the Lab Manager.