Duration: 3 Years

Shift Timing: 40 Hours/Week (Mon to Fri)

Job Description:

• The purpose of this position is to perform and train veterinary procedures for laboratory and research scientists and fellows and assist in therapeutic ultrasound procedures in experimental animals including mice, and rats; operate computer and laboratory equipment.
• aid in the development of Animal Care and Use Committee (ACUC) protocols.
• participate in the planning of and performing research studies; ordering and maintaining the necessary supplies for laboratory imaging use.
• demonstrate the proper use and methods associated with MRI scanners and associated software and develop new methods and procedures or substantially modify existing guidelines to resolve technical problems.

Role and Responsibilities:

• ???Study the principles of living systems, with a particular focus on the effects on animal biology, veterinary medicine, imaging techniques, and ancillary laboratory equipment, and up to date with the current Animal Care and Use Committee (ACUC) for appropriate care of animals. Maintain and update animal protocols for new and existing techniques.

• Prepares animals for experimental imaging and therapeutic studies including anesthesia, shave, Nair, injection catheters, physiological monitoring, retro-orbital infusion, intraarterial infusion, intracardiac injection, subcutaneous tumor implantation, intraperitoneal injection, surgical techniques
• implantation, intraperitoneal injection, surgical techniques, necropsies, blood and tissue sample collection, and proper disposal of animal carcasses.
• Maintain spreadsheet of experiment animal records and clinical care/observations.
• Maintains animal colonies.
• Handle, dissect and euthanize animals.
• Performs simple surgeries on animals.
• Handle experimental animals including tail vein injections, administration of anesthesia, and monitoring of animal viral signs.
• Prepares and analyzes animal tissues.
• Perform experiments and maintain primary tissues and cells in culture, utilizing sterile techniques.
• Perform a variety of specialized tissue fixation, embedding, sectioning, staining and immunohistochemistry, immunofluorescence techniques and perform analysis of animal tissues; prepare frozen slices and paraffin section, appropriately stained to demonstrate normal and abnormal structures and perform light and confocal microscopy.
• Performs molecular biology techniques.
• Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linked immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
• Performs immunohistochemistry on animal tissues.
• Work with staff to process tissue for immunohistochemistry.
• Analyzes experimental data.
• Update and maintain laboratory notebooks and databases; collect, quantitative analysis; data analysis, figure preparation, and image acquisition; maintain complete and detailed records of all experiments and interpret data.