We are seeking a highly motivated individual for a full-time Research Specialist position in Dr. Hun-Way Hwang's laboratory. Our current research focuses on the role of abnormal mRNA processing in human disease. We combine powerful in vivo high-throughput profiling methods and CRISPR technology with genetically modified mouse models of human disease to identify candidate mRNA isoforms that may contribute to disease pathogenesis in specific cell types in vivo. These candidate mRNA isoforms will then be further studied by in vitro and in vivo methods to reveal their biological functions and the molecular mechanisms regulating their processing, which will provide novel insights into the management and treatment of human disease. Our two recent publications describe how cancer cells switch mRNA isoforms to survive chemotherapy (https://doi.org/10.1016/j.celrep.2021.109815) and how hyperactive mTORC1 alters mRNA isoform expression in tuberous sclerosis complex (https://doi.org/10.7554/eLife.85036). For more information, visit www.hwangrnalab.org. This is a good opportunity for someone looking to gain research experience and benefit from direct mentorship by an experienced investigator. Our former technicians all went on to graduate or medical schools. Applicants should have a Bachelor's degree in Biology or related field. Significant undergraduate lab experience beyond coursework and familiarity with the basics of molecular biology and mammalian cell culture is desired.

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

- Maintain daily operation of the lab and mouse colonies- This will include: preparing common lab reagents, ordering, filing inventories, tracking ongoing projects, equipment maintenance, lab organization to comply with chemical, radiation, and biohazard standards, regular visits to the mouse facility, breeding, weaning and genotyping- Perform mammalian cell culture and molecular biology experiments including cell passage and maintenance, cell transfection, nucleic acid extraction, and conventional and real-time PCR.

This position is primarily sedentary. Incumbent may be required to travel back and forth between lab areas with occasional lifting of reagent containers and placing buffers and solutions on shelves.

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The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.