Position Summary
This position contributes to the design and delivery of research projects and collaborates with researchers to develop, execute and interpret research projects. Performs simple as well as complex laboratory and data collection and analysis techniques. This Research Specialist position is available in the laboratory of Dr Steven Roth, Department of Anesthesiology. Research projects in the lab examine rodent models of central retinal artery occlusion. In vitro studies use cell lines and primary neural cells prepared from rats or mice. Assays include mRNA isolation and qPCR analysis, biochemical assays to measure cell viability, Western blotting, cytokine assays, immunostaining of cells and tissue sections, quantitation of cell loss, and in vivo experiments on retinal function
Duties & Responsibilities

Isolation of RNA, real time PCR, treating rats with drugs, and monitoring clinical signs

Preparation and immunostaining of retina and brain sections, Western blotting, cytokine assays, maintenance of cell lines.

Perform in vitro studies in 6, 24, 96 or 384 well plate format, in vivo studies of retinal function.

Conduct statistical analysis and present data.

Other laboratory responsibilities include helping with ordering, stocking supplies, checking gas levels, general laboratory maintenance.

Perform other related duties and participate in special projects as assigned.