Slingshot Biosciences is looking for a Scientist to join the Cell Therapy Team to advance early-stage cell-based unit operations for in vivo cell therapy applications using cell mimics for CAR-T therapies. The successful candidate will work collaboratively across multiple teams within Slingshot Biosciences to develop and perform in vitro preclinical assays to evaluate CAR-T cell mimics phenotype and functionality: this includes designing and executing in vitro flow cytometry assays and cytokine secretion analyses to evaluate the potency of our engineered CAR-T cell mimics.

• Design and perform in vitro assays evaluating the potency and functional activity of engineered CAR-T cell mimics
• Perform routine cell culture to maintain various cell lines
• Develop and optimize cellular assays including co-culture assays for mechanistic investigation
• Establish strong in-house capabilities to perform in vitro experiments with high throughput
• Analyze data and communicate results clearly and effectively to program teams, senior leadership, and other colleagues
• Conduct and support in vivo studies to evaluate efficacy, mechanism of action, tolerability, and/or pharmacokinetics of novel therapeutics 
• Work cross-functionally with Scientists and Research Associates across departments to advance program objectives
• Maintain meticulous records of experimental methods, tool development and protocols

Requirements 

• Ph.D. degree (or equivalent) in Immunology, Cell Biology, or a related scientific discipline, with 0-2 years of postdoctoral experience in human T cell immunology or CAR-T cells
• Alternatively, M.S. degree (or equivalent) with 4 years of experience in the fields of human T cell immunology or CAR-T cells
• Experience with research scale cell and tissue culture techniques with primary cells or established cell lines 
• Experience with cell viability assays, cell-based fluorescence and chemiluminescence assays
• Proficiency and independence performing flow cytometry, cytokine analysis methods such as ELISA, MSD, EliSpot and other functional and phenotypic assays for in vitro characterization of T cells
• Experience with CAR-specific functional assays (cytotoxicity, cytokine release, proliferation) 
• Strong problem-solving skills, and demonstrated ability to apply rigorous and creative thinking to address complex research problems
• Strong organizational skills with ability to manage multiple priorities, and ability to work collaboratively and effectively in a team to achieve shared objectives
• Technical attention to detail, and experience in maintaining rigorous lab documentation
• Resourcefulness in overcoming challenges, and the ability to adapt and thrive in a fast-paced environment
• Ability to communicate results (verbal and written) effectively in a cross-functional team environment