Duties:
Design, plan and execute experiments in a timely and independent manner leveraging scientific and technical expertise to achieve defined objectives. Independently initiate and complete routine in vivo study duties such as dosing by standard routes, identification, health monitoring and sample collection as well as tissue processing. Initiate and carry out process for assay development, optimization and validation as required. Utilize molecular techniques, tissue immunostaining and multi-color flow cytometry to aid in the understanding of drug mode of action. Routine maintenance of laboratory equipment. Ordering of laboratory consumables to ensure continuous supplies. Work well in a highly matrixed environment and support multiple projects at the same time. Work independently with the capability to troubleshoot experimental procedures. Provide training and guidance to laboratory personnel. Communicate own work effectively orally and in writing (writing protocols, procedures, and lab notebook). Report and treat data with a high level of integrity and ethics. Comply with applicable regulations, performing all work in a safe manner and maintains proper records in accordance with SOPs and policies.
Skills:
Experience with animal handling, routine routes of injection, health monitoring, sample collection and tissue collection is required. Practical experience with general molecular biology techniques such as qPCR and nucleic acid extraction from tissues and cells is required. Practical experience with multi-color/ multi-parametric cytometry-based approaches, including cell preparation, staining and acquisition (highly desirable). Practical experience with histology procedures including embedding, cutting, and sectioning as well as immunostaining (highly desirable). Experience with ELISA, Western blot and cell culture with both mammalian cell lines and primary immune cells is a plus. Proficiency in Microsoft Office and familiarity with data analysis software (GraphPad, FlowJo, etc) is highly desirable.
Required Skills:

ANIMAL HANDLING

QPCR

FLOW CYTOMETRY

HISTOLOGY

ASSAY DEVELOPMENT
Additional Skills:

MOLECULAR BIOLOGY

WESTERN BLOT

ELISA

CELL CULTURE

LABORATORY
Proficiency with computers and familiarity with data analysis software (GraphPad, FlowJo