Laboratory TechnicianJob Summary: Medical Diagnostic Laboratories (MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses. MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS). MDL is looking for a Laboratory Technician. The Laboratory Technician is responsible for printing labels and attaching requisitions to the corresponding specimens. Essential Functions: Accessioning: Print labels and attach to requisitions and the corresponding specimensProperly accession multiple specimen typesTake photographs of all specimens receivedProperly identify any discrepant informationFile all paperwork in order and distribute to the appropriate QC binsProperly scan accessioned requisitionsScan 2-D barcode elution tubes used for extractionProperly locate and record temperatures for all necessary stations Manual Extraction: Perform Phenol/Chloroform manual extractions on biopsies and ticksProperly separate samples into their organic and inorganic layers via phenol/chloroform Properly perform quantitative analysis and hydrate all ticks and biopsies Robotic Extraction: Properly extract all samples types using a provided XG sheet as a templateProperly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagentsProperly dispose of all waste material when required; both hazardous and non-hazardousAttend to robotics to address any user interactions and/or robotic malfunctionsManually extract all necessary samples via commercial kits RNA Extraction: Perform manual RNA extractions via Qiagen kit for all necessary samplesPerform robotic RNA extractions via VX Reagents kit for all necessary samples PCR: Perform manual and robotic Conventional method PCR amplification Properly load and start thermocycler machinesPerform manual and robotic Real-time method PCR amplificationProperly load and start Rotor-gene thermocyclerPerform initial analysis on completed Real-time PCRPerform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic DispensorProperly load and start Bio-Rad CFX thermocyclerPerform initial analysis on completed Acoustic Real-Time PCR Gel Electrophoresis: Properly identify which completed PCRs from the thermocycler require gel electrophoresisProperly identify when to make and/or use 2% versus 2.5% gelsComplete blank gel template sheets to combine short PCR sheetsUse dye to stain all necessary samples with the appropriate volume on a test by test basisProperly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)Properly capture a visual image of the gel with the use of a SYNGENE UV gel readerBe able to troubleshoot any gel box or power source errors, as well as any gel reader errors Pyrosequencing Properly perform nested PCR prior to sequencingProperly load and run Sequencing Analysis reactions (SQA)Properly load and run Single Nucleotide Polymorphism reactions (SNP)Properly analyze all run data Micro-Biology/Cell Culture Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single coloniesProperly determine specific species present, and inoculate appropriate plate with said species on a quad plate preloaded with antibiotics, or an agar plate stamped with antibiotic discsProperly analyze growth plate to determine antibiotic susceptibility or resistanceFor GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic Chamber SystemIdentify and pick colonies for PCRPerform CAMP test and analyze for zones of hemolysis Next-Generation Sequencing Properly perform a series of nested PCRs for BRCA, HPV, and Cystic FibrosisProperly attach samples to appropriate bead mixturesProperly perform beads cleaning to remove unused dNTP’s and primers within the amplified PCR productProperly perform gel purification with the use of the Pippin Prep machineProperly load and operate multiple Life Technologies EquipmentProperly analyze all run data Additional Responsibilities: Performs other similar or related duties as required or assigned.Assures activities are conducted in a safe manner and materials are handled according to safety guidelines. Assures customer privacy is maintained at all times. Participates as a team member and is accountable for own work responsibilities.Assures information is delivered in a manner that is supportive, timely and understandable. Initiative is demonstrated to proactively diagnose and resolve problems. Productive work habits are consistently displayed. Accuracy and quality are maintained for all activities. Job Qualifications: General Knowledge, Skills, and Abilities (KSA’s) required 9-month Clinical Laboratory Technician training Physical Demands: Physical, Mental and Workplace Environment Conditions Ability to sit, stand, walk and perform repetitive motion (manual pipette)Proficient visual and audio skills Workplace Conditions: Workplace Environment Conditions Laboratory settingUse of personal protective equipment may be necessaryMay be exposed to chemicals, blood/body fluids Education and Certifications: Bachelor Degree in Biology, Chemistry, or related science field Visit us at https://www.mdlab.com/ Medical Diagnostic Laboratories is a member of Genesis Global Group, an equal opportunity employer.