Current Employees: If you are a current Staff, Faculty or Temporary employee at the University of Miami, please click here to log in to Workday to use the internal application process. To learn how to apply for a faculty or staff position using the Career worklet, please review this tip sheet. Sr. Research Laboratory Technician – Yeager Lab, The Frost Institute for Chemistry and Molecular Science The Yeager lab is intrigued by biological questions at the interface between cell biology and structural biology. How do membrane channels open and close? How are signals transmitted across a cellular membrane when an extracellular ligand binds to a membrane receptor? How do viruses attach and enter host cells, replicate and assemble infectious particles? We use high resolution electron cryo-microscopy (cryo-EM) and image processing to explore the molecular design of large, multicomponent supramolecular assemblies. Biological specimens are quick frozen in a physiological state to preserve their native structure and functional properties. A special advantage of this rapid-freezing method is that we can trap and image dynamic states of functioning macromolecular assemblies, such as open and closed states of membrane channels and viruses actively transcribing RNA. Three-dimensional density maps are obtained by digital image processing of the high-resolution electron micrographs. The rich detail in the maps reveals the structural organization of complex biological structures that can be related to the functional properties of such assemblies. The University of Miami is offering an excellent job opportunity for a Senior Research Technician position at the Frost Institute for Chemistry and Molecular Science within the laboratory of Dr. Mark Yeager. We are looking for a talented Sr. Research Laboratory Technician who will serve as key personnel on our current research projects. This individual will conduct research experiments in accordance with laboratory and safety protocols and will collaboratively and independently perform research laboratory techniques and procedures. The Sr. Research Laboratory Technician performs advanced technical activities related to laboratory research projects within the department. Specifically, the Sr. Research Laboratory Technician aids scientists by performing experiments, monitoring and recording study findings. CORE JOB FUNCTIONS Maintains laboratory cell cultures, including mammalian, bacterial and insect cells. Under general supervision performs complex laboratory techniques including, but not limited to recombinant protein purification, DNA purification, flow cytometry. Cleans, sanitizes, maintains, and operates laboratory equipment and glassware. Maintains laboratory supply inventory and assists with ordering. Performs data collection, data management and analysis using laboratory/computer equipment. Uses biological safety cabinets, balances, pH meter, laboratory instruments such as laminar flow hoods, centrifuges, FPLC systems, flow cytometer. Maintains accurate records of procedures used to conduct experiments. Contributes to report writing and graphical presentation of data. Completes all required documentation for laboratory requests. Schedules laboratory work based on project priorities to meet deadlines. Adheres to University and unit-level policies and procedures and safeguards University assets. This list of duties and responsibilities is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary. CORE QUALIFICATIONS Education: High school diploma or equivalent. Bachelor’s degree in biological sciences or related discipline (physics, chemistry) preferred. Certification and Licensing: Not Applicable Experience: Minimum 3 years relevant experience working in a research laboratory performing laboratory procedures in the areas of biochemistry, molecular biology, microbiology or cell biology. Any appropriate combination of relevant education, experience and/or certifications may be considered. Knowledge, Skills and Attitudes: Skill in completing assignments accurately and with attention to detail. Ability to analyze, organize and prioritize work under pressure while meeting deadlines. Ability to process and handle confidential information with discretion. Ability to work evenings, nights, and weekends as necessary. Commitment to the University’s core values. Ability to work independently and/or in a collaborative environment. Proficiency in computer software (i.e., Microsoft Office). The University of Miami is an Equal Opportunity Employer - Females/Minorities/Protected Veterans/Individuals with Disabilities are encouraged to apply. Applicants and employees are protected from discrimination based on certain categories protected by Federal law. Click here for additional information. Job Status: Full time Employee Type: Staff Pay Grade: A6 The mission of the University of Miami is to transform lives through education, research, innovation, and service. A vibrant and diverse academic and healthcare community, the University of Miami and the University of Miami Health System ("UHealth") have rapidly progressed to become one of the nation’s top research universities and academic medical centers in the nation. The University comprises 12 schools and colleges serving undergraduate and graduate students in more than 350 majors and programs. Visit welcome.miami.edu/about-um to learn more about our points of pride. The University ranks No. 55 on U.S. News & World Report’s 2022 Best Colleges list and ranked No. 49 in the 2022 Wall Street Journal/Times Higher Education College Rankings. Bascom Palmer Eye Institute is ranked the #1 eye hospital in the USA and offers some of the nation's premiere eye doctors to treat every eye condition for adults. Sylvester Comprehensive Cancer, part of the Miller School of Medicine, received the prestigious National Cancer Institute designation in 2019. The University of Miami and UHealth have also ranked among the Forbes Best Employers and Best Employers for Women on several occasions, most recently in 2022. Transforming lives With more than 17,000 faculty and staff, the University strives for excellence, and is driven by a powerful mission to transform and impact the lives of its students, patients, members of the community, and people across the globe. The University is committed to fostering a culture of belonging, where everyone feels valued and has the opportunity to add value. Through values of Diversity, Integrity, Responsibility, Excellence, Compassion, Creativity, and Teamwork (DIRECCT) the U community works together to create an environment driven by purpose, excellence, community, and service.