Our client is a biotechnology company in Maryland is looking to hire a Research Laboratory Technician with 3 years of cell culture and laboratory experience as an independent contractor. The candidate will be responsible for the performance of operations in support of starting/establishing, growth and production of naïve stem cells, hESCs, iPSCs and differentiated cells from tissue samples under GMP conditions. These functions may include expansion and maintenance of ESCs and propagation and differentiation of ESCs into other cell lines in culture flasks and bioreactors (2L - 10L) using aseptic techniques, cryopreservation of cells, preparation of media and reagents, filtration, inventory management, collecting and analyzing data and preparing reports.

Responsibilities

• Interact and communicate effectively with supervisor, and other site personnel.

• Assist in the preparation and analysis of laboratory experiments, as requested.

• Perform cell culture and related bioassays of cell cultures.

• Perform cell/protein characterization – western blot, PCR, flow cytometry, and read/analyze data.

• Perform preliminary research, information gathering, and information packaging.

• Assist the laboratory team with collection of data and data input.

• Document all correspondence and communication pertinent to the research.

• Adhere to Occupational Safety and Health Administration (OSHA), Good Laboratory Practices (GLP), GMP, and infection control procedures.

• Responsible for cleaning, disinfecting and maintenance of all clean rooms and equipment.

• Assist with receipt and inspection of raw materials, lab ordering and stocking, as requested.

• Process and ship all samples according to regulations and protocol requirements.

• Maintain and update files of all applicable regulatory documentation.

Qualifications

• 3-5 years of relevant experience in a biomedical, biotechnology or research laboratory.

• 3 years of expansion and maintenance of hESCs, iPSCs in cell culture.

• Experience differentiating ESCs into other cell lines is a plus.

• Demonstrate competence and professionalism in oral and written communication.

• Must be organized, attentive to detail, and possess a positive, friendly, and professional demeanor.

• Must be flexible with changing priorities.

• Computer skills - at least intermediate level experience with Excel and other Microsoft Office Software

• Experience with data management and processing.

• Familiarity with medical and scientific terminology and abbreviations.

Education

• Bachelor's degree in Biology or Life Sciences required.