What are the responsibilities and job description for the Bioanalytical Mass Spectrometry Scientist position at Franklin Bio Lab?
Franklin Biolabs emerges from the esteemed legacy of Dr. Jim Wilson's laboratory, a beacon of innovation in vector technology for over three decades. Building on this rich heritage, Franklin Biolabs is poised to redefine the landscape of the global genetic medicines industry. As a Contract Research Organization (CRO), Franklin Biolabs offers a comprehensive suite of services spanning from discovery through clinical manufacturing. Our goal is to empower our partners by providing unparalleled solutions that drive the advancement of genetic medicines. Leveraging decades of expertise and a commitment to excellence, we bridge the gap between groundbreaking research and ensuring that transformative therapies reach those who need them most.
We are seeking a highly motivated and experienced Bioanalytical Mass Spectrometry Scientist to support discovery and preclinical development programs through the development, optimization, and execution of LC-MS/MS-based bioanalytical assays. The ideal candidate will have strong hands-on expertise with both high-resolution and tandem quadrupole mass spectrometry platforms, particularly Thermo Orbitrap and Agilent Triple Quadrupole systems, and experience analyzing diverse therapeutic modalities including proteins, peptides, small molecules, and antisense oligonucleotides (ASOs).
Key Responsibilities
- Develop, optimize, validate, and troubleshoot quantitative and qualitative bioanalytical LC-MS/MS methods for:
- Proteins and biologics
- Peptides
- Small molecules
- Antisense oligonucleotides (ASOs)
- Perform sample preparation and extraction from complex biological matrices including:
- Mouse plasma, serum, tissues, and CSF
- Non-human primate (NHP) plasma, serum, tissues, and biofluids
- Operate, maintain, and troubleshoot:
- Thermo Orbitrap LC-MS/MS systems
- Agilent Triple Quadrupole LC-MS/MS systems
- Design and execute bioanalytical workflows supporting PK, TK, biodistribution, biomarker, and metabolism studies.
- Develop sensitive and robust quantitative assays using high-resolution MS and MRM-based methods.
- Analyze and interpret complex mass spectrometry datasets using vendor and third-party software tools.
- Collaborate cross-functionally with DMPK, pharmacology, translational biology, and toxicology teams.
- Maintain detailed experimental records and prepare technical reports, SOPs, and study summaries.
- Ensure compliance with internal quality standards and regulatory guidelines where applicable.
- Stay current with emerging technologies and advancements in bioanalytical mass spectrometry.
Education and Experience:
- Ph.D. or M.S. in Analytical Chemistry, Biochemistry, Pharmaceutical Sciences, Molecular Biology, or related scientific discipline.
- 3 years (industry preferred) of hands-on experience in bioanalytical mass spectrometry.
- Demonstrated expertise in LC-MS/MS method development and optimization.
- Strong experience with:
- Thermo Orbitrap platforms
- Agilent Triple Quadrupole systems
- Proven ability to detect and quantify proteins, peptides, small molecules, and ASOs in biological matrices.
- Experience working with mouse and non-human primate (NHP) samples.
- Familiarity with chromatographic techniques including reverse-phase, ion-pairing, and HILIC separations.
- Strong understanding of bioanalytical assay validation principles.
- Excellent problem-solving, organizational, and communication skills.
- Experience supporting oligonucleotide therapeutics programs.
- Knowledge of regulated bioanalysis (GLP/GCLP environment).
- Experience with hybrid ligand-binding/MS workflows.
- Familiarity with automation platforms and high-throughput sample preparation.
- Experience with metabolite identification and biomarker analysis.
- Proficiency with data analysis software such as:
- Xcalibur
- MassHunter
- Skyline
- Analyst or equivalent tools
Knowledge, Skills and Abilities:
- Development of highly sensitive quantitative LC-MS/MS assays.
- High-resolution accurate mass (HRAM) method development.
- MRM assay development on triple quadrupole instruments.
- Detection and characterization of low-abundance analytes in complex matrices.
- Troubleshooting ion suppression, matrix effects, and extraction recovery issues.
- Strong understanding of PK/PD and biodistribution applications.
Work Environment
- Fast-paced, collaborative research environment supporting early discovery through preclinical development.
- Opportunity to contribute to cutting-edge therapeutic platforms including oligonucleotide and peptide therapeutics.
- This position requires full-time on-site presence at our Discovery Labs location in King of Prussia, PA.
Vision:
- Ability to distinguish between different colors (normal color vision).
- Ability to see clearly at various distances.
- Adequate near vision for reading documents and spreadsheets.
- Ability to focus and maintain concentration in tasks for extended periods.
- Ability to work in environments with varying light conditions.
Company Benefits
- 401(k)
- 401(k) Matching
- Health insurance
- Dental insurance
- Vision insurance
- Health Savings Account and Flexible Spending Account
- Paid Time-Off
- Company Paid Holidays
- Personal Floating Holidays
- Short-Term and Long-Term Disability
- Life and AD&D insurance (Group & Voluntary)