75438BRJob Posting Title:Research Assistant I-Nigrovic LaboratoryDepartment:Medicine-Allergy and Immunology AutoReqId:75438BRStatus:Full-TimeStandard Hours per Week:40 Job Posting Category:ResearchJob Posting Description:A research assistant position is available with a flexible start date for June, July, August or September 2024 within the laboratory of Dr. Peter Nigrovic, Chief of Immunology at Boston Childrens Hospital. The individual will join a team studying adaptive immune mechanisms of autoimmune diseases. We apply cutting-edge approaches in transcriptomics, immunology, molecular biology, and gene editing and state-of-the-art microscopy on human patient samples and mouse models of autoimmune diseases. The research assistant will conduct studies in tissue culture, microscopy, immunology and molecular biology techniques, animal husbandry and surgical procedures. The candidate may interact with patients and participate actively in lab meetings, discussions and presentations. A 2-year commitment is preferred. Training will be provided in all skills, rendering the position suitable for a self-motivated individual considering medical or graduate school.

This Research Assistant will be responsible for:

Tissue culture

Cellular and molecular immunology assays including but not limited to qPCR, flow cytometry, confocal and intra-vital 2-photon microscopy, western blot, etc.

Animal husbandry and colony maintenance

Consenting and collecting patient samples

Processing, organizing and cryopreserving patient samples

According to prescribed protocols and assigned schedules, performs a variety of routine laboratory experiments and procedures, utilizing standard laboratory equipment, recording and checking results for completeness and accuracy.

Assists in troubleshooting procedures if experimental results are unexpected or quality of specimens is poor.

Performs the following techniques typically required for the laboratory/study:

Maintains records of results and methodologies in a log of work performed.

Maintains equipment, lab area, and supplies in a neat professional manner.

Sterilize and clean glassware, and prepare media and other materials using appropriate procedures.

Inventories supplies, alerts supervisor to needed supplies, or mixes standard reagents.Processes order for supplies and/or animals.

Attends courses in safety procedures for handling, storage, and disposal of biohazardous, flammable, corrosive, and toxic materials.

Carries out all laboratory activities in compliance with applicable regulations and protocols.

Conducts literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I.

Attends courses, lectures, and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers and technicians to exchange information.

Contributes to the team within and between departments and organizations.

In order to qualify you must have:

A BS degree in Biology/Molecular Biology/Immunology or related field.

Flexible working hours during weekdays AND weekends as needed.

Past research experience in tissue culture, cellular and molecular immunology and/or mouse work preferred but not required.

Excellent communication, organization, time management, and record-keeping a must.

Boston Childrens Hospital offers competitive compensation and unmatched benefits including flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.

Office/Site Location:BostonRegular, Temporary, Per Diem:Regular Remote Eligibility :Onsite Only