Scientist (Immunology)

Position – Scientist (Immunology)

Location – Cambridge, MA

Contract – 06 Months with a possibility of extension

Position Summary

• Join the Systems Immunology Lab to generate large-scale single-cell perturbational datasets.
• Utilize functional genomics and single-cell transcriptomics in collaboration with a small, cross-functional team.

Key Responsibilities

• Generate custom lentiviral libraries and maintain extensive cell cultures.
• Create edited cell pools via lentiviral transduction and arrayed electroporation.
• Develop, modify, and scale protocols for specific cell lines and cell types.
• Produce single-cell data using commercial platforms, including 10x Genomics workflows.
• Organizational Relationships
• Report to a Lead Scientist within the Systems Immunology Lab.
• Collaborate with multidisciplinary experimental teams on a project basis.

Education & Experience

• Bachelor’s or Master’s degree in Biology, Immunology, or a related field.
• Minimum 3 years of relevant research experience.

Technical Skills

• 3 years of hands-on experience with:
• Standard cell culture techniques.
• Primary immune cell handling, isolation, and in vitro stimulation.
• Maintenance of cell lines.
• Molecular biology techniques: lentiviral infection, qPCR, western blot, flow cytometry.

Bonus: Experience with pooled CRISPR-based screening (CRISPRi, CRISPRa, gene editing) in immune cells.

Physical Requirements

Typical bench work, including sitting, pipetting, and lifting items up to ~10 lbs.

Interview Process

Round 1: Microsoft Teams video interview (camera on)

Round 2: Onsite interview (2 hours with stakeholders)