RA/SRA – HTP Antibody Expression and Screening

RA/SRA – HTP Antibody Expression and Screening

About the job

The heart of Prellis Bio’s strategy is the combination of novel, cutting edge methods in machine learning, biology at scale and next gen antibody discovery that address long-standing industry-wide problems in the drug development pipeline. Prellis Biologics uses proprietary technology to 3D print human lymph node organoids enabling the rapid & diverse discovery of human antibody therapeutic candidates for a range of applications. To drive this forward, we are assembling an incredible team of discovery biologists, computational scientists, and protein scientists who want to make a difference to this important problem.

Position Summary

We are seeking a Research Associate / Senior Research Associate – HTP Antibody Expression to grow our high-throughput (HTP) expression capabilities and accelerate our biotherapeutic discovery efforts. You will be responsible for:

• Spearheading our HTP protein expression platform, delivering high quality antibody from our EXIS and AI/ML platforms for early screening.
• Leading our mid-scale cloning and expression operations of select, key antibodies needed for downstream testing.

The ideal candidate will thrive in a dynamic environment and play a critical role in delivering life-changing therapeutics.

Responsibilities

• Perform small-scale HTP Expi293 and ExpiCHO transient expressions using automated and semi-automated systems.
• Express human IgGs, bispecifics, Fc-fusion proteins.
• Maintain cell lines for HTP and large scale antibody production.
• Optimize antibody expression for maximal scale, speed and yield.
• Perform bacterial cloning and mid-scale expression of key antibodies of interest.
• Maintain accurate ELN documentation.
• Communicate results and support cross-team workflows.

Qualifications

• BS in Biochemistry, Molecular Biology, or a related field with 3 years of relevant industry experience.
• Demonstrated experience in HTP antibody expression.
• Experience with automated and semi-automated expression systems. (BioMek, KingFisher, Lynx, Integra ViaFlo).
• Experience with bacterial cloning, especially Gibson Assembly and Golden Gate Assembly.
• Experience with multiple antibody formats (human IgG, bispecific, Fc Fusion).
• Excellent sterile technique.
• Strong understanding of therapeutic antibody discovery process.
• Strong organizational skills, attention to detail, and ability to drive multiple projects.
• Demonstrated experience in collaborating across various scientific disciplines in a dynamic environment.
• Keen interest in pushing the envelope on HTP production scale and capacity.

Bonus Experience

• Familiarity with automated HTP Protein A based purification protocols.
• Familiarity with protein QC on LC/MS and aSEC.
• Familiarity with cell-based HTP screening methods.

What you can expect of us

As we work to develop treatments that take care of others, we also work to care for your professional and personal growth and well-being. From our competitive benefits to our collaborative culture, we’ll support your journey every step of the way.

The expected annual salary range for this role in the U.S. is posted. Actual salary will vary based on several factors including but not limited to, relevant skills, experience, and qualifications.

Application deadline

Prellis does not have an application deadline for this position; we will continue accepting applications until we receive a sufficient number or select a candidate for the position.

Prellis Bio is an equal opportunity employer. All applicants will be considered for employment without attention to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran or disability status.

We believe diversity, equity, and inclusion need to be at the foundation of our culture. We work hard to bring together diverse teams–grounded in a wide range of expertise and life experiences–and work even harder to ensure those teams thrive in inclusive, growth-oriented environments supported by equitable company and team practices. All candidates can expect equitable treatment, respect, and fairness throughout the interview process.