NGS Scientist

Details:

Title: NGS Scientist

Duration: 12 Months Contract

Location: Boston, MA 02210

Pay Rate: $57.28/Hr on W2

Summary:

At Client, we unite caring with discovery to make life better for people around the world. We are a global healthcare leader headquartered in Indianapolis, Indiana. Our 35,000 employees around the world work to discover and bring life-changing medicines to those who need them, improve the understanding and management of disease, and give back to our communities through philanthropy and volunteerism. We give our best effort to our work, and we put people first. We’re looking for people who are determined to make life better for people around the world.

Job Description:

Client is seeking an NGS Contractor to join our team in Boston, MA.

Come join the Cardiometabolic Research (CMR) team at client! We are focused on the discovery and early phase clinical development of biologic, small molecule, oligonucleotide, and cell therapies for treatment of diabetes, obesity, cardiovascular disease, and associated metabolic disorders. The Data Science team within CMR is seeking someone with wide-ranging experience in next-generation sequencing library preparation to help achieve our research goals. The successful candidate will be responsible for RNA-seq, scRNA-seq, snRNA-seq, and other types of -seq library preparations for all CMR groups. This will be a cross-therapeutic area role encompassing opportunities to impact science in the Boston team.

Job Responsibilities:

• Execute NGS library preparation (e.g., bulk RNA, scRNA, snRNA sequencing), quantification, and sequencing workflows to support the CMR team.
• Internalize and develop new NGS workflows to support evolving needs.
• Develop and adhere to standard operating procedures for workflows to ensure consistent production of high-quality data.
• Work in the sequencing space cross-functionally, with the ability to manage multiple requests and queues.
• Deliver NGS data with clear expectations of timelines to multiple teams and promptly update stakeholders of run completion.
• Effectively manage inventory of DNA, RNA, libraries, and sequencing reagents.
• Perform additional related tasks (i.e., RNA extraction, qPCR/ddPCR) as needed to help the team meet goals and deliverables.
• Maintain detailed documentation of NGS run performance and scientific work in an electronic notebook.
• Participate in assessment of sample prep and sequencing workflows at other sites (Indianapolis and/or contract research organizations) to enable flex capacity when demand is high (no travel required).

Job Requirements:

• B.S. and/or M.S. in biological or biochemical sciences (or a related field) with 3-5 years of laboratory research experience.
• Molecular biology mastery of nucleic acid extraction and purification, quantification, NGS library preparation/QC, and qPCR.
• Strong background in NGS technologies and their applications, including quantification, sequencing, and data QC (preferably Illumina). Direct experience with RNA-seq (bulk) and amplicon-seq.
• Direct experience with single-cell and single-nucleus RNA-seq library preparation is required. Candidates must be familiar with multiple scRNA-seq/snRNA-seq platforms (e.g., 10X Genomics Chromium, Illumina PIP-seq, ScaleBio, or Parse Biosciences).

Additional Skills/Preferences:

• Familiarity with CRISPR-based gene editing and high-throughput screening is a plus.
• Experience with automation is a plus (Mantis, Integra systems, Liquid Handlers, SPTLabtech Firefly, Tecan, etc).
• Strong organizational, time management, and communication skills.
• Experience with documentation in electronic notebook software.