Immunology Scientist

Job Title: Immunology Scientist
Location: Cambridge, MA
Hours/Schedule: Monday–Friday, 8 am to 5 pm
Type: Contract (6 months)

The Scientist will join the Systems Immunology Lab within an Inflammation & Immunology research unit to generate large-scale single-cell perturbational datasets. This role focuses on functional genomics and single-cell transcriptomics, working hands-on at the bench and collaborating closely with a small, multidisciplinary research team.

• Generate custom lentiviral libraries and maintain large-scale cell cultures.

• Produce edited cell pools using lentiviral transduction and arrayed electroporation.

• Develop, optimize, and customize protocols to support diverse cell lines and cell types at scale.

• Generate single-cell datasets using commercial workflows, including 10x Genomics platforms.

• Perform standard molecular biology and cell-based assays to support functional genomics studies.

• Collaborate with multidisciplinary experimental teams on a per-project basis.

• Document experimental procedures, results, and observations in accordance with laboratory standards.

• Bachelor’s or Master’s degree in Biology, Immunology, or a related scientific field.

• 3 years of hands-on laboratory experience.

• Strong experience with cell culture techniques, including primary immune cell handling, isolation, and in vitro stimulation.

• Experience maintaining cell lines and working with large-scale cultures.

• Hands-on experience with molecular biology techniques, including lentiviral infection, qPCR, western blotting, and flow cytometry.

• Ability to perform routine bench work, including pipetting and lifting items up to approximately 10 lbs.

• Direct experience with pooled cell screening approaches such as CRISPRi, CRISPRa, or other in vitro gene-editing methods.

• Experience generating or working with single-cell transcriptomics data.

System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan.