Biologist (Electron Microscopy)

Position Objective: The Biologist will provide support services to satisfy the overall operational objectives of the National Institute on Aging (NIA) within the National Institutes of Health (NIH). The primary objective is to provide research services and deliverables through performance of support services for the Confocal Imaging Facility core of the NIA IRP.

Duties And Responsibilities

• Study basic principles of plant and animal life, such as origin, relationship, development, anatomy, and function
• Identify, classify, and study structure, behavior, ecology, physiology, nutrition, culture, and distribution of plant and animal species
• Proficiency in Confocal Microscopy image acquisition and analysis 1
• Proficiency in Immunohistochemistry IHC and ICC and immunofluorescence IF techniques 4
• Functional knowledge of super-resolution microscopy techniques 5
• Sample preparation for transmission electron microscopy (TEM); fixation, resin embedding, ultrathin sectioning, post staining, immunogold labeling and negative staining. 2 2
• Preparation of solutions and chemicals 3
• Troubleshooting and optimizing laboratory protocols and instrumentation.
• User training and support.
• Operate laboratory equipment to analyze samples
• Analyze laboratory findings
• Operation of the TEM and support equipment
• Responsible for a clean and organized laboratory.
• User training and support.
• Program and use computers to store, process, and analyze data
• Interpret research or operational data
• Record research or operational data
• Pursue the research goals of the laboratory by conducting wet-lab works
• Work with staff on performing various experiments and procedures
• Harvest and collect mouse embryos, tissues and organs
• Perform histological techniques, including tissue and or whole-mount in situhybridization and immunohistochemistry
• Prepare and organize various lab samples
• Conduct molecular biological analyses
• Provide a broad range of technical assistance to laboratory members
• Maintain laboratory supplies, equipment and experimental records
• Provide support for ordering reagents and equipment required for the laboratory
• Work products and documents related to conducting wet-lab works including harvesting and collecting mouse embryos, tissues and organs; perform histological techniques; conduct various molecular biological analyses.
• Monthly Work products and documents related to maintaining the laboratory’s supplies, equipment and experimental records.
• Clean Equipment, Run Validation, Inspect Equipment, Meet with lab members to present updates, Work products and documents related to training and supervising researchers in confocal microscopy