Senior Associate Scientist/ Principal Research Associate, Antibody and Protein Engineering

Job Title: Associate Scientist/ Sr. Associate Scientist Antibody and Protein Engineering

Reports To:  Scientist II, Antibody Engineering

Position:

Mediar Therapeutics is a clinical stage biotechnology company developing therapeutics to halt, or even reverse, fibrosis and restore long-term organ function. The platform and pipeline are based on an emerging class of novel targets- fibrotic mediators – that play key roles in modulating myofibroblast biology and the development of fibrosis chronically damaged organs. Mediar was founded by Mass General Brigham Ventures, Massachusetts General Hospital and Brigham & Women’s Hospital and has laboratory and office space in Boston.

We are seeking a Senior Associate scientist, or Principal Research Associate to join our protein engineering team.  This position offers the opportunity to impact multiple steps of the discovery and engineering process for biotherapeutic lead generation including the expression, purification, and characterization of immunogens, panning reagents and the therapeutic candidates themselves.  The successful candidate will be a self-motivated scientist with demonstrated communication and organizational skills who can work collaboratively in a dynamic environment.

Role Responsibilities:

• Plan and execute laboratory experiments supporting antibody and protein engineering programs, with appropriate guidance from project leads.
• Execute expression and purification of monoclonal and bispecific antibodies, including optimization and troubleshooting of protein production and purification workflows.
• Perform biochemical and biophysical characterization of antibody and protein molecules using techniques including:
  • Bio-Layer Interferometry (BLI)
  • ELISA
  • HPLC/FPLC
  • SDS-PAGE
• Evaluate antibody/protein quality, purity, and stability to identify potential technical or developability concerns.
• Analyze and independently interpret experimental data, drawing scientifically sound conclusions, and recommendations of appropriate next steps.
• Maintain mammalian cell culture (HEK293 and CHO platforms)
• Maintain accurate and timely documentation of experimental procedures, results, and conclusions in an electronic laboratory notebook.
• Troubleshoot experimental challenges and independently identify and implement appropriate solutions.
• Present experimental results and scientific conclusions clearly during project team meetings and to the broader scientific organization.
• Collaborate closely with scientists across antibody engineering, translational research, and other cross-functional teams to advance project objectives.
• Contribute to development and continuous improvement of laboratory methods, assays, and workflows.
• Maintain a high standard of laboratory organization, data integrity, and compliance with applicable laboratory procedures.
• Onsite – 5 days/ week

Required Qualifications:

• B.S. or M.S. degree in Biochemistry, Molecular Biology, Protein Sciences, or a related discipline, with relevant industry experience.
• Associate Scientist Level – mimimum of 2 years of industry R&D experience (and B.S)
• Senior Associate Scientist Level – minimum of 5 years of industry R&D experience (with B.S) or 3 years of industry R&D experience (with M.S)
• Demonstrated hands-on experience with mammalian cell culture, recombinant protein expression, and protein purification.
• Experience with bispecific antibodies or engineered antibody formats is highly desirable.
• Hands-on experience with biochemical and biophysical characterization techniques, particularly BLI, ELISA, HPLC/FPLC, and SDS-PAGE.
• Strong ability to independently execute experiments, troubleshoot technical issues, and manage multiple experimental activities with appropriate prioritization.
• Demonstrated ability to analyze and interpret experimental data rather than simply execute established protocols.
• Strong attention to detail and commitment to high-quality experimental documentation and data integrity.
• Excellent scientific communication skills, including the ability to present experimental results and conclusions to both project teams and broader scientific audiences.
• Ability to work effectively in a collaborative, fast-paced, and highly cross-functional research environment.

Preferred Qualifications:

• Extensive experience with biochemical and biophysical characterization techniques, particularly BLI, ELISA, HPLC/FPLC, and SDS-PAGE.
• Experience in high throughput screening assays using FACS is a plus.
• Experience in protein purification using AKTA-FPLC instruments.
• Proficiency in mammalian cell culture includes media prep, cell line maintenance, cryopreservation, and transfections.

Our Culture & Values

At Mediar, we foster a collaborative, mission-driven environment where every team member contributes to advancing transformative therapies for patients with fibrotic diseases. Our core values are Curiosity, Community, and Integrity. These values guide everything we do, from scientific innovation to how we work together as a team.

Equal Employment Opportunity

Mediar is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability, veteran status or any other classification protected under applicable law.  Mediar also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. 

Ranges: 

Associate Scientist: 80K - 85K

Senior Associate Scientist: 85K - 97K