What are the responsibilities and job description for the Scientist - Molecular Biologist position at Jacaranda consulting?
Key Responsibilities
- Develop, optimize and validate molecular biology methods for parameters such as: VCN, gene editing proficiency, infectious titer, genomic titer, replication competent lentivector, residual DNA, etc
- Understand viral vector designs and develop appropriate molecular assays to support assay development in gene and cell therapies.
- Develop and optimize CRISPR-based screens using genome-wide and targeted gRNA libraries
- Perform molecular biology techniques, including cloning, DNA plasmid purification (small & large-scale) and in vitro transcription to generate assay controls, PCR (qPCR, dPCR) and ELISA-based assays.
- Analyze and interpret data, prepare technical reports, and present findings.
- Collaborate with cross-functional teams to support assay development, contract research service or QC testing.
Essential Qualifications
- A PhD or Master’s degree in Life Sciences, Molecular Biology, Biotechnology, Biochemistry or related field.
- Required: Extensive, hands-on experience in PCR (qPCR and dPCR) and NGS assays and bioinformatic analysis
- Is required.
- A robust understanding of molecular assay performance criteria, applicable to fluorescent qPCR/dPCR assays (including multiplexed assays), according to applicable regulatory guidance.
- Knowledge and experience with retroviral, transposon, or retroelement molecular biology
- Ability to plan, organize and multi-task to complete assignments in an efficient manner.
- A sense of urgency, speed, persistence, and organization in a dynamic and exciting work environment.
Job Type: Full-time
Pay: $85,000.00 - $100,000.00 per year
Benefits:
- 401(k)
- Commuter assistance
- Dental insurance
- Health insurance
- Paid time off
- Stock options
- Vision insurance
Application Question(s):
- Do you have Strong experience in digital PCR, qPCR development, validation?
- Do you have strong experience in molecular cloning?
- Do you have experience working with various mammalian cell lines?
- Do you have experience working with lentivirus or retrovirus?
- Do you have experience with CRISPR-cas9 gene editing?
Education:
- Master's (Preferred)
Work Location: In person
Salary : $85,000 - $100,000