What are the responsibilities and job description for the Molecular Biologist position at GAP Solutions, Inc.?
Position Objective: The Molecular Biologist will provide support services to the National Institute of Allergy and Infectious Diseases within the National Institutes of Health.
Duties And Responsibilities
Duties And Responsibilities
- Provide support for molecular/structural biology projects, as well as immunogen testing and analysis for an effective HIV vaccine and vaccines for other pathogens. 1
- Prepare protein probes for B cell sorting and antibody isolation projects. 2
- Documents research findings and maintains accurate logs and records of all experimental activities and outcomes.
- Perform B cell sorting using multicolor flow cytometry and single B cell sequencing to identify antibodies of interest. 3
- Provide expression of viral glycoproteins, antibodies, immunogens and virus-like particles using mammalian expression systems; provide expression of immunogens in alternative expression systems.
- Provide DNA vector manipulation including expression vector design, DNA/protein sequence analysis, DNA recombination, mutagenesis and plasmid preparation.
- Provide biochemical analysis of proteins.
- Maintain and generate stable cell lines.
- Maintain tissue culture facility (maintenance of cell lines, cell culture mediums and supplies).
- Work products and documents related to expressing proteins from eukaryotic expression systems.
- Work products and documents related to maintaining tissue culture facility.
- Work products and documents related to providing support for molecular/structural biology projects including the design of an effective HIV vaccine; carry out B cell culture.
- Work products and documents related to providing analysis of proteins.
- Maintain tissue culture facility (maintenance of cell lines, cell culture mediums and supplies).
Salary : $130,000 - $142,000