Scientist

Job Description: Pay Range: $55.02hr - $60.02hr Responsibilities:XXgn and construct expression vectors using advanced molecular cloning techniques including Gibson assembly, Golden Gate cloning, restriction enzyme-based cloning, and site-directed mutagenesis.Develop, optimize, and implement automated workflows for high-throughput vector construction and DNA assembly on liquid handling platforms.Generate, validate, and bank vector constructs for expression of candidate biotherapeutic molecules in mammalian cell systems.Perform molecular characterization of cell lines and constructs using techniques such as ddPCR, Sanger sequencing, and restriction mapping.Support NGS library preparation for genomic and transcriptomic analysis of CHO cell lines.Optimize and troubleshoot molecular biology protocols to improve efficiency and reproducibility.Prepare technical reports and presentations to communicate progress and data.Basic Qualifications:PhD (0-3 years) in Molecular Biology, Cell Biology, Biochemistry, Bioengineering, or related discipline.Extensive hands-on experience with molecular cloning techniques including Gibson assembly, Golden Gate cloning, restriction cloning, and PCR-based methods.Demonstrated expertise in DNA construct design, vector engineering, and plasmid preparation.Experience with genetic characterization methods such as ddPCR, qPCR, gel electrophoresis, and DNA sequencing analysis.Proficiency in designing and optimizing molecular biology workflows for automation on liquid handling platforms (Hamilton, Tecan, or similar)Strong technical background in molecular biology with excellent attention to detail and troubleshooting skills.Self-motivated with excellent organization, time-management, and communication skills.Maintain detailed electronic laboratory notebooks and documentation of all experimental procedures.Demonstrated ability to work as member of a team and adhere to timelines.Preferred Qualifications:Mammalian cell culture experience in a pharmaceutical or biotechnology setting, particularly with CHO cells.Experience with high-throughput screening and clone selection strategiesFamiliarity with bioinformatic tools for DNA sequence analysis, primer design, and in silico cloning (Snapgene, Geneious, Benchling, Primer3 or similar)Knowledge of CHO cell line development processes and recombinant protein expression.Experience using Oxford Nanopore Technologies in NGS workflowsBasic programming skills in R or Python for data analysis and visualizationExperience with Bash/command line scripting for bioinformatics workflows and data processing.Experience with automated colony picking and clone tracking systemsFlexibility to adjust to a fast-paced, goal-oriented environment and willingness to expand and broaden skillset.

Salary : $55 - $60