What are the responsibilities and job description for the Downstream Purification Scientist position at Careers Integrated Resources Inc?
Title: Downstream Purification Scientist
Location: Boston, MA- Onsite
Duration: 12 Months (Possible extension – based on performance)
Shift: Standard
Payrate: $39 - $47/hr on W2
Key Responsibilities:
Location: Boston, MA- Onsite
Duration: 12 Months (Possible extension – based on performance)
Shift: Standard
Payrate: $39 - $47/hr on W2
Key Responsibilities:
- Execute purification processes including CsCl density gradient ultracentrifugation, affinity chromatography (e.g., AVB, CaptureSelect), ion-exchange chromatography (AEX, CEX), and other polishing steps.
- Operate AKTA systems (Avant, Pure, or Pilot) for small- to large-scale purification runs.
- Perform TFF operations for buffer exchange and concentration of intermediate or final products.
- Support experimental design, data collection, and documentation following established procedures and GMP/GLP best practices.
- Assist in troubleshooting and optimization of purification processes.
- Analyze in-process and purified samples using relevant analytical methods (e.g., UV, SDS-PAGE, ddPCR, ELISA, or HPLC).
- Collaborate cross-functionally with upstream, analytical, and manufacturing teams.
- Maintain lab equipment, manage buffers and column inventory, and ensure compliance with safety and documentation standards.
- We are seeking a highly motivated Contractor – Purification Scientist to join our Downstream Process Development team.
- The successful candidate will contribute to the purification and characterization of viral vectors, biologics, or recombinant proteins to support process development and manufacturing activities.
- The ideal candidate has hands-on experience in Cesium chloride (CsCl) density gradient ultracentrifugation, ion-exchange chromatography, affinity purification, and tangential flow filtration (TFF), with a strong understanding of purification principles and data analysis.
- Education: Master’s degree in biology, biochemistry, chemical engineering, biotechnology, or related field.
- Experience: Minimum 2 years of hands-on purification experience in an academic or industry setting.
- Technical Skills:
- Strong knowledge and practical experience with CsCl density gradient ultracentrifugation, ion-exchange, and affinity chromatography.
- Familiarity with buffer preparation.
- Understanding of basic analytical methods for product characterization.
- Strong attention to detail and excellent documentation habits.
- Effective communication and teamwork skills.
- Ability to manage multiple tasks and meet project timelines.
- Experience in AAV or other viral vector CsCl density gradient and column purification.
- Experience with AKTA systems and Unicorn software.
Salary : $39 - $47