Research Associate I-III IN VITRO

WHAT WE DO:

Calidi Biotherapeutics is a clinical-stage immuno-oncology company with proprietary technology that is revolutionizing the utilization of oncolytic viruses through an allogeneic cell-based delivery system.  Calidi Biotherapeutics is advancing this potent stem cell and oncolytic virus  combination for use in multiple difficult-to-treat oncology indications. Our team is also developing a universal cell delivery system to protect, amplify and potentiate oncolytic viruses currently in development.

Job Overview/Summary:

The IN VITRO Research Associate, performs research and/or development in collaboration with others for projects. Makes detailed observations, analyzes data and interprets results. Investigates, creates and develops new methods and technologies for project advancement.

Essential Duties and Responsibilities: 

• Generate immunophenotyping and immune cell functional data using techniques such as multicolor flow cytometry, ELISA, and xCELLigence RTCA. 
• Isolate protein, DNA, and RNA from cell cultures and tissues; perform gene and protein analysis using qPCR and Western blot. 
• Maintain primary and established cell lines to support in vivo studies. 
• Assist with general cell culture for the production of oncolytic viruses, including herpes simplex virus, vaccinia virus, and/or adenovirus. 
• Contribute to the generation of recombinant therapeutic viruses. 
• Support the in vivo team as needed, including animal handling and sample collection. 
• Analyze and organize experimental data with strong attention to detail. 
• Assist with laboratory maintenance, inventory, and cleaning tasks. 
• Adhere to EH&S guidelines, participate in mandatory safety training, and follow SOPs to ensure study integrity and compliance. 
• Perform other duties as assigned.

Skills/Qualifications:

• 2 years of experience in a biotechnology company with broad knowledge and experience in cell and molecular biology methods; Immuno-Oncology field is preferred.
• Experience with mamallan cell culture is required. 
• Experience in immune cell based assay (ELISA, MSD, cytotoxicity assays) is required. Strong hands-on experience in acquiring and analyzing flow cytometry data (multi-parameter flow cytometry, intracellular, extracellular staining, flowjo analysis) is highly desirable. 
• Experience with mouse handling, including restraint, injection (IP, IV, SC), and tissue collection is highly desirable.
• Experience in a range of in vitro cellular/molelecular biology techniques (cell culture, cloning, qPCR, Western blot, immunohistochemistry, microscopy) is preferred.  
• Excellent data organization, analysis, and troubleshooting skills using electronic lab notebook. 
• Dedication to working in a team-oriented environment to contribute to shared project.
• Strong work ethic, self-motivation, and ability to multi-task in a fast-paced work environment.
• Strong knowledge of MS Word, Outlook, PowerPoint, Excel, Graphad Prism, Flowjo. 

Education, Certificates, Licenses, Registrations:

• B.S. or higher in life science, or related field

Mathematical Skills: 

• Ability to work with mathematical concepts such as calculation of dilutions and statistical analyses. 
• Ability to apply concepts such as fractions, percentages, ratios, and proportions to practical situations.

REASONING ABILITY: 

• Ability to define problems, collect data, establish facts, and draw valid conclusions.

Language Skills:

• Excellent communication skills with ability to effectively communicate with other research teams
• Ability to read and understand technical procedures
• Ability to write protocols and reports
• Ability to effectively present data and respond to questions from co-workers

The Perks:

• Breakroom snacks and beverages 
• Competitive Total Rewards package (Medical, Dental, Vision) offered to full-time employees
• Stock options

Work Location:

Calidi Biotherapeutics employment provides the opportunity to work in beautiful San Diego, California only minutes from the nearest beach.

EOE and Accommodation:

We value diversity and are proud to be an Equal Opportunity and Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, protected veteran status, pregnancy, citizenship, marital status, gender expression, genetic information, political affiliation, or any other characteristic protected by law. Calidi Biotherapeutics provides reasonable accommodation in job application procedures for qualified individuals with disabilities and disabled veterans. If you need accommodation in connection with the recruiting process due to a disability, you may include this information in your cover letter. If you are selected to interview for a position, you may also request an accommodation with our team directly. 

Notice to Agency and Search Firm Representatives

Calidi Biotherapeutics is not accepting unsolicited resumes from agencies and/or search firms for this job posting. Resumes submitted to any Calidi Biotherapeutics employee by a third-party agency and/or search firm without a valid written & signed search agreement, will become the sole property of Calidi Biotherapeutics. No fee will be paid if a candidate is hired for this position as a result of an unsolicited agency or search firm referral. 

EMPLOYER’S RIGHTS: 

This job description does not list all the duties of the job. You may be asked by supervisors or managers to perform other duties. You will be evaluated in part based upon your performance of the tasks listed in this job description.

 The employer has the right to revise this job description at any time. The job description is not a contract for employment, and either you or the employer may terminate employment at any time, for any reason.