Cryo Electron Microscopy Specialist

• We need someone who is experienced in using running a cryo-electron microscope and can design experiments, collect the data from the experiments, and analyze/interpret the results.
• We are not looking to a histotechnician who solely has experience preparing samples, but the person needs to be able to do the sample prep as well as the cryo-EM/ET work

Roles / Responsibilities

• Perform sample preparation for both single-particle analysis (SPA) cryo-EM and cryo-electron tomography (cryo-ET).
• Collect, analyze, and interpret cryo-EM and cryo-ET dataset.
• Collaborate with other scientists to design and execute experiments.
• Support the lab with operation of side-entry cold-holder
• Train and mentor junior staff and scientists in cryo-EM and cryo-ET techniques.
• Work with staff to conduct research, including planning and conducting experiments and data analysis.
• Locate and review the literature pertinent to projects to identify the most applicable protocols and methods; perform experiments in accordance with protocols.
• Collect, organize, and record experimental data for publication and future reference by others.
• Prepare analyses and reports for publication and presentation; participate in laboratory meetings.
• Organize and maintain laboratory, including inventory and ordering of supplies and equipment; organize and maintain stocks of reagents.
• Provide orientation and training to new staff.
• Provide training and mentoring to trainees and colleagues when needed.
• Prepare datasets for publication and deposit in controlled-access databases.

Requirements

• Bachelor’s degree; preference for MSc/Ph.D. degree in Biological Sciences or other related discipline.
• Minimum of two years of experience in a research laboratory environment.
• Minimum of 2 years of experience in the cryoEM field.
• Experience with sample preparation for both SPA and Cryo-ET.
• Experience in computational analysis of cryo-EM data
• Familiarity with state-of-the-art cryo-EM software packages and high-performance computing units (HPC).
• Current working knowledge of the software and hardware developments in cryo-EM, and limitations of the technique.
• Interest in participating in research projects.
• Strong communication skills, both oral and written.
• Excellent analytical, organizational and time management skills.
• Strong analytical and problem-solving skills.
• Excellent communication and teamwork skills.